Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system
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چکیده
منابع مشابه
investigating the integration of translation technologies into translation programs in iranian universities: basis for a syllabus design in translation technology
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Site-specific chromosomal integration of large synthetic constructs
We have developed an effective, easy-to-use two-step system for the site-directed insertion of large genetic constructs into arbitrary positions in the Escherichia coli chromosome. The system uses lambda-Red mediated recombineering accompanied by the introduction of double-strand DNA breaks in the chromosome and a donor plasmid bearing the desired insertion fragment. Our method, in contrast to ...
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We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty of the tool described here is the inclusion of a minimal bacterial attachment site (attB(min)), two mutated loxP sequences (lox66 and lox71) a...
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Integration of A Lipase Gene into the Bacillus subtilis Chromosome: Recombinant Strains Without Antibiotic Resistance Marker
A new system is presented for the generation of recombinant <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2011
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-011-9397-y